Introducing proteomics in the undergraduate curriculum: A simple 2D gel electrophoresis exercise with serum proteins

Behavior of the Serum proteins in starch-agar-gel electrophoresis

How to use 2D gel electrophoresis in plant proteomics.

Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays.Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an attractive choice when a proteomics strategy...

behavior of the serum proteins in starch-agar-gel electrophoresis

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Starch-gel electrophoresis of rat-serum proteins.

In 1955, Smithies (1) described a procedure for the separation of human serum proteins by horizontal zone electrophoresis in starch gels. The separation of zones was improved by a later modification to a vertical electrophoresis in which the sample was inserted in the gel, free from supporting medium (2). With these methods, it has been demonstrated that many more protein fractions can be separ...

Distribution of the serum proteins of Syrian hamster as revealed by Starch gel electrophoresis